1JJR

The Three-Dimensional Structure of the C-terminal DNA Binding Domain of Human Ku70

Summary for 1JJR

• Entry DOI: 10.2210/pdb1jjr/pdb
• NMR Information: BMRB: 4941
• Descriptor: THYROID AUTOANTIGEN (1 entity in total)
• Functional Keywords: dna repair protein, protein-dna interaction, ku70, solution structure, dna binding protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 16969.52

Authors

Zhang, Z.,Chen, Y. (deposition date: 2001-07-09, release date: 2001-10-03, Last modification date: 2024-05-22)

Primary citation

Zhang, Z.,Zhu, L.,Lin, D.,Chen, F.,Chen, D.J.,Chen, Y.
The three-dimensional structure of the C-terminal DNA-binding domain of human Ku70.
J.Biol.Chem., 276:38231-38236, 2001

PubMed Abstract: The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA double-strand break repair pathway in mammalian cells. Interaction of Ku with DNA is central for the functions of Ku. Ku70, which is mainly responsible for the DNA binding activity of the Ku heterodimer, contains two DNA-binding domains. We have solved the solution structure of the Ku80-independent DNA-binding domain of Ku70 encompassing residues 536-609 using nuclear magnetic resonance spectroscopy. Residues 536-560 are highly flexible and have a random structure but form specific interactions with DNA. Residues 561-609 of Ku70 form a well defined structure with 3 alpha-helices and also interact with DNA. The three-dimensional structure indicates that all conserved hydrophobic residues are in the hydrophobic core and therefore may be important for structural integrity. Most of the conserved positively charged residues are likely to be critical for DNA recognition. The C-terminal DNA-binding domain of Ku70 contains a helix-extended strand-helix motif, which occurs in other nucleic acid-binding proteins and may represent a common nucleic acid binding motif.

PubMed: 11457852

Experimental method

SOLUTION NMR