1JJP

A(GGGG) Pentad-Containing Dimeric DNA Quadruplex Involving Stacked G(anti)G(anti)G(anti)G(syn) Tetrads

1JJP の概要

• エントリーDOI: 10.2210/pdb1jjp/pdb
• 分子名称: 5'-D(*GP*GP*GP*AP*GP*GP*TP*TP*TP*GP*GP*GP*AP*T)-3' (1 entity in total)
• 機能のキーワード: a(gggg) pentad, dimeric quadruplex, double chain reversal loop, g(anti)g(anti)g(anti)g(syn) tetrads, v-shaped loop and scaffold, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 8863.75

構造登録者

Zhang, N.,Gorin, A.,Majumdar, A.,Kettani, A.,Chernichenko, N.,Skripkin, E.,Patel, D.J. (登録日: 2001-07-09, 公開日: 2001-09-19, 最終更新日: 2024-05-22)

主引用文献

Zhang, N.,Gorin, A.,Majumdar, A.,Kettani, A.,Chernichenko, N.,Skripkin, E.,Patel, D.J.
V-shaped scaffold: a new architectural motif identified in an A x (G x G x G x G) pentad-containing dimeric DNA quadruplex involving stacked G(anti) x G(anti) x G(anti) x G(syn) tetrads.
J.Mol.Biol., 311:1063-1079, 2001

PubMed Abstract: We report the results of an NMR study of unlabeled and uniformly (13)C,(15)N-labeled d(G(3)AG(2)T(3)G(3)AT) in 100 mM NaCl, conditions under which it forms a dimeric quadruplex containing several new topological features. The DNA oligomer chain in each symmetry-related monomer subunit undergoes three sharp turns to form a compact domain, with all the purine bases involved in pairing alignments. The first turn is of the double chain reversal type, the second is of the edgewise type, and the third represents a new alignment, the V-shaped type. Each monomer of the dimeric quadruplex contains two stacked G(anti) x G(anti) x G(anti) x G(syn) tetrads, one of which forms a newly identified A x (G x G x G x G) pentad, through sheared G.A mismatch formation. There is a break in one of the four G-G columns that link adjacent G x G x G x G tetrads within each monomer. This architectural interruption is compensated by a new topological feature of quadruplex architecture, the V-shaped scaffold. The missing G-G column results in an opening that could facilitate insertion of planar ligands into the quadruplex. The dimeric interface contains stacked A.(G.G.G.G) pentads, with each pentad containing four bases from one monomer and a syn G1 from the partner monomer. Several potential ligand-binding pockets, positioned towards either end of the folded architecture, were identifiable in a surface view of the solution structure of the dimeric d(G(3)AG(2)T(3)G(3)AT) quadruplex.

PubMed: 11531340
DOI: 10.1006/jmbi.2001.4916

実験手法

SOLUTION NMR