1JJD

NMR structure of the Cyanobacterial Metallothionein SmtA

1JJD の概要

• エントリーDOI: 10.2210/pdb1jjd/pdb
• NMR情報: BMRB: 5147
• 分子名称: METALLOTHIONEIN, ZINC ION (2 entities in total)
• 機能のキーワード: zinc finger, zinc cluster, metallothionein, metal binding protein
• 由来する生物種: Synechococcus elongatus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5878.94

構造登録者

Sadler, P.J.,Robinson, N.J. (登録日: 2001-07-04, 公開日: 2001-08-22, 最終更新日: 2024-05-01)

主引用文献

Blindauer, C.A.,Harrison, M.D.,Parkinson, J.A.,Robinson, A.K.,Cavet, J.S.,Robinson, N.J.,Sadler, P.J.
A metallothionein containing a zinc finger within a four-metal cluster protects a bacterium from zinc toxicity.
Proc.Natl.Acad.Sci.USA, 98:9593-9598, 2001

PubMed Abstract: Zinc is essential for many cellular processes, including DNA synthesis, transcription, and translation, but excess can be toxic. A zinc-induced gene, smtA, is required for normal zinc-tolerance in the cyanobacterium Synechococcus PCC 7942. Here we report that the protein SmtA contains a cleft lined with Cys-sulfur and His-imidazole ligands that binds four zinc ions in a Zn(4)Cys(9)His(2) cluster. The thiolate sulfurs of five Cys ligands provide bridges between the two ZnCys(4) and two ZnCys(3)His sites, giving two fused six-membered rings with distorted boat conformations. The inorganic core strongly resembles the Zn(4)Cys(11) cluster of mammalian metallothionein, despite different amino acid sequences, a different linear order of the ligands, and presence of histidine ligands. Also, SmtA contains elements of secondary structure not found in metallothioneins. One of the two Cys(4)-coordinated zinc ions in SmtA readily exchanges with exogenous metal ((111)Cd), whereas the other is inert. The thiolate sulfur ligands bound to zinc in this site are buried within the protein. Regions of beta-strand and alpha-helix surround the inert site to form a zinc finger resembling the zinc fingers in GATA and LIM-domain proteins. Eukaryotic zinc fingers interact specifically with other proteins or DNA and an analogous interaction can therefore be anticipated for prokaryotic zinc fingers. SmtA now provides structural proof for the existence of zinc fingers in prokaryotes, and sequences related to the zinc finger motif can be identified in several bacterial genomes.

PubMed: 11493688
DOI: 10.1073/pnas.171120098

実験手法

SOLUTION NMR