1JHB

HUMAN GLUTAREDOXIN IN FULLY REDUCED FORM, NMR, 20 STRUCTURES

1JHB の概要

• エントリーDOI: 10.2210/pdb1jhb/pdb
• 分子名称: GLUTAREDOXIN (1 entity in total)
• 機能のキーワード: glutaredoxin, oxidoreductase, electron transport
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P35754
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11787.71

構造登録者

Sun, C.,Bushweller, J.H. (登録日: 1998-02-17, 公開日: 1998-08-26, 最終更新日: 2024-05-22)

主引用文献

Sun, C.,Berardi, M.J.,Bushweller, J.H.
The NMR solution structure of human glutaredoxin in the fully reduced form.
J.Mol.Biol., 280:687-701, 1998

PubMed Abstract: The determination of the nuclear magnetic resonance (NMR) solution structure of fully reduced human glutaredoxin is described. A total of 1159 useful nuclear Overhauser effect (NOE) upper distance constraints and 187 dihedral angle constraints were obtained as the input for the structure calculations for which the torsion angle dynamics program DYANA has been utilized followed by energy minimization in water with the AMBER force field as implemented in the program OPAL. The resulting 20 conformers have an average root-mean-square deviation value relative to the mean coordinates of 0.54 A for all the backbone atoms N, Calpha and C', and of 1.01 A for all heavy atoms. Human glutaredoxin consists of a four-stranded mixed beta-sheet composed of residues 15 to 19, 43 to 47, 72 to 75 and 78 to 81, and five alpha-helices composed of residues 4 to 9, 24 to 34, 54 to 65, 83 to 91, and 94 to 100. Comparisons with the structures of Escherichia coli glutaredoxin-1, pig liver glutaredoxin and human thioredoxin were made. Electrostatic calculations on the human glutaredoxin structure and that of related proteins provide an understanding of the variation of pKa values for the nucleophilic cysteine in the active site observed among these proteins. In addition, the high-resolution NMR solution structure of human glutaredoxin has been used to model the binding site for glutathione and for ribonucleotide reductase B1 by molecular dynamics simulations.

PubMed: 9677297
DOI: 10.1006/jmbi.1998.1913

実験手法

SOLUTION NMR