1JH3

Solution structure of tyrosyl-tRNA synthetase C-terminal domain.

1JH3 の概要

• エントリーDOI: 10.2210/pdb1jh3/pdb
• 関連するPDBエントリー: 2TS1
• NMR情報: BMRB: 5070
• 分子名称: TYROSYL-TRNA SYNTHETASE (1 entity in total)
• 機能のキーワード: aminoacyl-trna synthetase, anticodon-arm binding domain, ligase
• 由来する生物種: Geobacillus stearothermophilus
• 細胞内の位置: Cytoplasm: P00952
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12001.54

構造登録者

Guijarro, J.I.,Pintar, A.,Prochnicka-Chalufour, A.,Guez, V.,Gilquin, B.,Bedouelle, H.,Delepierre, M. (登録日: 2001-06-27, 公開日: 2002-03-20, 最終更新日: 2024-05-29)

主引用文献

Guijarro, J.I.,Pintar, A.,Prochnicka-Chalufour, A.,Guez, V.,Gilquin, B.,Bedouelle, H.,Delepierre, M.
Structure and Dynamics of the Anticodon Arm Binding Domain of Bacillus stearothermophilus Tyrosyl-tRNA Synthetase
Structure, 10:311-317, 2002

PubMed Abstract: The structure of a recombinant protein, TyrRS(delta4), corresponding to the anticodon arm binding domain of Bacillus stearothermophilus tyrosyl-tRNA synthetase, has been solved, and its dynamics have been studied by nuclear magnetic resonance (NMR). It is the first structure described for such a domain of a tyrosyl-tRNA synthetase. It consists of a five-stranded beta sheet, packed against two alpha helices on one side and one alpha helix on the other side. A large part of the domain is structurally similar to other functionally unrelated RNA binding proteins. The basic residues known to be essential for tRNA binding and charging are exposed to the solvent on the same face of the molecule. The structure of TyrRS(delta4), together with previous mutagenesis data, allows one to delineate the region of interaction with tRNATyr.

PubMed: 12005430
DOI: 10.1016/S0969-2126(02)00699-8

実験手法

SOLUTION NMR