1JFP

Structure of bovine rhodopsin (dark adapted)

1JFP の概要

• エントリーDOI: 10.2210/pdb1jfp/pdb
• 関連するPDBエントリー: 1eds 1edv 1edw 1edx 1fdf
• 分子名称: rhodopsin, RETINAL (2 entities in total)
• 機能のキーワード: g-protein coupled receptor, signaling protein, membrane protein
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Membrane; Multi-pass membrane protein: P02699
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39315.89

構造登録者

Yeagle, P.L.,Choi, G.,Albert, A.D. (登録日: 2001-06-21, 公開日: 2001-10-05, 最終更新日: 2022-02-23)

主引用文献

Yeagle, P.L.,Choi, G.,Albert, A.D.
Studies on the structure of the G-protein-coupled receptor rhodopsin including the putative G-protein binding site in unactivated and activated forms.
Biochemistry, 40:11932-11937, 2001

PubMed Abstract: Activation of G-protein coupled receptors (GPCR) is not yet understood. A recent structure showed most of rhodopsin in the ground (not activated) state of the GPCR, but the cytoplasmic face, which couples to the G protein in signal transduction, was not well-defined. We have determined an experimental three-dimensional structure for rhodopsin in the unactivated state, which shows good agreement with the crystal structure in the transmembrane domain. This new structure defines the cytoplasmic face of rhodopsin. The G-protein binding site can be mapped. The same experimental approach yields a preliminary structure of the cytoplasmic face in the activated (metarhodopsin II) receptor. Differences between the two structures suggest how the receptor is activated to couple with transducin.

PubMed: 11570894
DOI: 10.1021/bi015543f

実験手法

SOLUTION NMR