1JFD

STRUCTURE OF INORGANIC PYROPHOSPHATASE

1JFD の概要

• エントリーDOI: 10.2210/pdb1jfd/pdb
• 分子名称: INORGANIC PYROPHOSPHATASE, SULFATE ION (3 entities in total)
• 機能のキーワード: hydrolase, acid anhydride hydrolase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A7A9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39386.79

構造登録者

Oganesyan, V.,Avaeva, S.M.,Huber, R.,Harutyunyan, E.H. (登録日: 1997-05-31, 公開日: 1997-12-03, 最終更新日: 2024-02-07)

主引用文献

Avaeva, S.,Kurilova, S.,Nazarova, T.,Rodina, E.,Vorobyeva, N.,Sklyankina, V.,Grigorjeva, O.,Harutyunyan, E.,Oganessyan, V.,Wilson, K.,Dauter, Z.,Huber, R.,Mather, T.
Crystal structure of Escherichia coli inorganic pyrophosphatase complexed with SO4(2-). Ligand-induced molecular asymmetry.
FEBS Lett., 410:502-508, 1997

PubMed Abstract: The three-dimensional structure of inorganic pyrophosphatase from Escherichia coli complexed with sulfate was determined at 2.2 A resolution using Patterson's search technique and refmed to an R-factor of 19.2%. Sulfate may be regarded as a structural analog of phosphate, the product of the enzyme reaction, and as a structural analog of methyl phosphate, the irreversible inhibitor. Sulfate binds to the pyrophosphatase active site cavity as does phosphate and this diminishes molecular symmetry, converting the homohexamer structure form (alpha3)2 into alpha3'alpha3". The asymmetry of the molecule is manifested in displacements of protein functional groups and some parts of the polypeptide chain and reflects the interaction of subunits and their cooperation. The significance of re-arrangements for pyrophosphatase function is discussed.

PubMed: 9237692
DOI: 10.1016/S0014-5793(97)00650-9

実験手法

X-RAY DIFFRACTION (2.2 Å)