1JEW

CRYO-EM STRUCTURE OF COXSACKIEVIRUS B3(M STRAIN) WITH ITS CELLULAR RECEPTOR, COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR (CAR).

1JEW の概要

• エントリーDOI: 10.2210/pdb1jew/pdb
• 関連するPDBエントリー: 1COV 1F5W 1KAC
• 分子名称: COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR, COXSACKIEVIRUS CAPSID, COAT PROTEIN VP1, COXSACKIEVIRUS CAPSID, COAT PROTEIN VP2, ... (5 entities in total)
• 機能のキーワード: coxsackievirus b3, cvb3, car, cryo-em structure, icosahedral virus, virus-receptor complex, virus/receptor
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 107193.61

構造登録者

Rossmann, M.G.,He, Y. (登録日: 2001-06-19, 公開日: 2001-10-03, 最終更新日: 2024-02-07)

主引用文献

He, Y.,Chipman, P.R.,Howitt, J.,Bator, C.M.,Whitt, M.A.,Baker, T.S.,Kuhn, R.J.,Anderson, C.W.,Freimuth, P.,Rossmann, M.G.
Interaction of coxsackievirus B3 with the full length coxsackievirus-adenovirus receptor.
Nat.Struct.Biol., 8:874-878, 2001

PubMed Abstract: Group B coxsackieviruses (CVB) utilize the coxsackievirus-adenovirus receptor (CAR) to recognize host cells. CAR is a membrane protein with two Ig-like extracellular domains (D1 and D2), a transmembrane domain and a cytoplasmic domain. The three-dimensional structure of coxsackievirus B3 (CVB3) in complex with full length human CAR and also with the D1D2 fragment of CAR were determined to approximately 22 A resolution using cryo-electron microscopy (cryo-EM). Pairs of transmembrane domains of CAR associate with each other in a detergent cloud that mimics a cellular plasma membrane. This is the first view of a virus-receptor interaction at this resolution that includes the transmembrane and cytoplasmic portion of the receptor. CAR binds with the distal end of domain D1 in the canyon of CVB3, similar to how other receptor molecules bind to entero- and rhinoviruses. The previously described interface of CAR with the adenovirus knob protein utilizes a side surface of D1.

PubMed: 11573093
DOI: 10.1038/nsb1001-874

実験手法

ELECTRON MICROSCOPY (22 Å)