1JES

Crystal Structure of a Copper-Mediated Base Pair in DNA

1JES の概要

• エントリーDOI: 10.2210/pdb1jes/pdb
• 関連するPDBエントリー: 1BNA 2ZNA 3ZNA
• 分子名称: 5'-D(*CP*GP*CP*GP*(DPY)P*AP*TP*(DRP)P*CP*GP*CP*G)-3', COPPER (II) ION (3 entities in total)
• 機能のキーワード: cu, copper, dna, z-dna, dodecamer, duplex, unnatural, designed
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7423.83

構造登録者

Atwell, S.,Meggers, E.,Spraggon, G.,Schultz, P.G. (登録日: 2001-06-18, 公開日: 2001-11-23, 最終更新日: 2024-04-03)

主引用文献

Atwell, S.,Meggers, E.,Spraggon, G.,Schultz, P.G.
Structure of a Copper-Mediated Base Pair in DNA
J.Am.Chem.Soc., 123:12364-12367, 2001

PubMed Abstract: Stable and selective DNA base pairing by metal coordination was recently demonstrated with nucleotides containing complementary pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) bases (Meggers, E.; Holland, P. L.; Tolman; W. B.; Romesberg, F. E.; Schultz, P. G. J. Am. Chem. Soc. 2000, 122, 10714-10715). To understand the structural consequences of introducing this novel base pair into DNA we have solved the crystal structure of a duplex containing the metallo-base pair. The structure shows that the bases pair as designed, but in a Z-DNA conformation. The structure also provides a structural explanation for the B- to Z-DNA transition in this duplex. Further solution studies demonstrate that the metallo-base pair is compatible with Z- or B-DNA conformations, depending on the duplex sequence.

PubMed: 11734038
DOI: 10.1021/ja011822e

実験手法

X-RAY DIFFRACTION (1.5 Å)