1JE8

Two-Component response regulator NarL/DNA Complex: DNA Bending Found in a High Affinity Site

1JE8 の概要

• エントリーDOI: 10.2210/pdb1je8/pdb
• 関連するPDBエントリー: 1A04 1RNL
• 分子名称: 5'-D(*CP*GP*TP*AP*CP*CP*CP*AP*TP*TP*AP*AP*TP*GP*GP*GP*TP*AP*CP*G)-3', Nitrate/Nitrite Response Regulator Protein NARL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: protein-dna complex, two-component response regulator, helix-turn-helix, dna bending, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 64616.06

構造登録者

Maris, A.E.,Sawaya, M.R.,Kaczor-Grzeskowiak, M.,Jarvis, M.R.,Bearson, S.M.D.,Kopka, M.L.,Schroder, I.,Gunsalus, R.P.,Dickerson, R.E. (登録日: 2001-06-15, 公開日: 2002-09-27, 最終更新日: 2024-10-09)

主引用文献

Maris, A.E.,Sawaya, M.R.,Kaczor-Grzeskowiak, M.,Jarvis, M.R.,Bearson, S.M.,Kopka, M.L.,Schroder, I.,Gunsalus, R.P.,Dickerson, R.E.
Dimerization allows DNA target site recognition by the NarL response regulator.
Nat.Struct.Biol., 9:771-778, 2002

PubMed Abstract: Two-component signal transduction systems are modular phosphorelay regulatory pathways common in prokaryotes. In the co-crystal structure of the Escherichia coli NarL signal output domain bound to DNA, we observe how the NarL family of two-component response regulators can bind DNA. DNA recognition is accompanied by the formation of a new dimerization interface, which could occur only in the full-length protein via a large intramolecular domain rearrangement. The DNA is recognized by the concerted effects of solvation, van der Waals forces and inherent DNA deformability, rather than determined primarily by major groove hydrogen bonding. These subtle forces permit a small DNA-binding domain to perturb the DNA helix, leading to major DNA curvature and a transition from B- to A-form DNA at the binding site, where valine on the recognition helix interacts unexpectedly with the polar major groove floor.

PubMed: 12352954
DOI: 10.1038/nsb845

実験手法

X-RAY DIFFRACTION (2.12 Å)