1JDH

CRYSTAL STRUCTURE OF BETA-CATENIN AND HTCF-4

1JDH の概要

• エントリーDOI: 10.2210/pdb1jdh/pdb
• 分子名称: BETA-CATENIN, hTcf-4 (3 entities in total)
• 機能のキーワード: beta-catenin, tcf4, protein-protein complex, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm : P35222; Nucleus, PML body : Q9NQB0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61831.24

構造登録者

Graham, T.A.,Ferkey, D.M.,Mao, F.,Kimelman, D.,Xu, W. (登録日: 2001-06-13, 公開日: 2001-12-05, 最終更新日: 2024-02-07)

主引用文献

Graham, T.A.,Ferkey, D.M.,Mao, F.,Kimelman, D.,Xu, W.
Tcf4 can specifically recognize beta-catenin using alternative conformations.
Nat.Struct.Biol., 8:1048-1052, 2001

PubMed Abstract: Accumulation of the Wnt pathway effector beta-catenin is a hallmark of a number of cancers, including colon cancer. As beta-catenin accumulates in the cell, it forms a complex with Tcf family transcription factors and activates the transcription of several critical genes involved in cell proliferation. Because Tcf4 is the predominant Tcf factor present in colon cancer cells, drugs that specifically disrupt the beta-catenin-Tcf4 complex could be useful in treating colon cancers. Earlier structural and biochemical studies demonstrated that the central region of the beta-catenin binding domain of Tcf is essential for anchoring Tcf to beta-catenin via two conserved lysines in beta-catenin (called the charged 'buttons'). Here we report the crystal structure of a beta-catenin-Tcf4 complex at 2.0 A resolution. Our structural and mutagenesis studies show that Tcf4 docks specifically to beta-catenin using several distinct conformations in its essential central region. These conformations allow different glutamate residues in the central region of Tcf4 to form a salt bridge with the same critical charged button, Lys 312 of beta-catenin. We propose that this interaction may be the first event in beta-catenin-Tcf4 recognition.

PubMed: 11713475
DOI: 10.1038/nsb718

実験手法

X-RAY DIFFRACTION (1.9 Å)