1JBW

FPGS-AMPPCP-folate complex

1JBW の概要

• エントリーDOI: 10.2210/pdb1jbw/pdb
• 関連するPDBエントリー: 1FGS 1JBV
• 分子名称: FOLYLPOLYGLUTAMATE SYNTHASE, MAGNESIUM ION, DIPHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER, ... (5 entities in total)
• 機能のキーワード: fpgs folate amppcp ternary complex, ligase
• 由来する生物種: Lactobacillus casei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47786.24

構造登録者

Sun, X.,Cross, J.A.,Bognar, A.L.,Baker, E.N.,Smith, C.A. (登録日: 2001-06-06, 公開日: 2001-09-19, 最終更新日: 2024-12-25)

主引用文献

Sun, X.,Cross, J.A.,Bognar, A.L.,Baker, E.N.,Smith, C.A.
Folate-binding triggers the activation of folylpolyglutamate synthetase.
J.Mol.Biol., 310:1067-1078, 2001

PubMed Abstract: Folic acid is an essential vitamin for normal cell growth, primarily through its central role in one-carbon metabolism. Folate analogs (antifolates) are targeted at the same reactions and are widely used as therapeutic drugs for cancer and bacterial infections. Effective retention of folates in cells and the efficacy of antifolate drugs both depend upon the addition of a polyglutamate tail to the folate or antifolate molecule by the enzyme folylpolyglutamate synthetase (FPGS). The reaction mechanism involves the ATP-dependent activation of the free carboxylate group on the folate molecule to give an acyl phosphate intermediate, followed by attack by the incoming L-glutamate substrate. FPGS shares a number of structural and mechanistic details with the bacterial cell wall ligases MurD, MurE and MurF, and these enzymes, along with FPGS, form a subfamily of the ADP-forming amide bond ligase family. High-resolution crystallographic analyses of binary and ternary complexes of Lactobacillus casei FPGS reveal that binding of the first substrate (ATP) is not sufficient to generate an active enzyme. However, binding of folate as the second substrate triggers a large conformational change that activates FPGS and allows the enzyme to adopt a form that is then able to bind the third substrate, L-glutamate, and effect the addition of a polyglutamate tail to the folate.

PubMed: 11501996
DOI: 10.1006/jmbi.2001.4815

実験手法

X-RAY DIFFRACTION (1.85 Å)