1JBJ

CD3 Epsilon and gamma Ectodomain Fragment Complex in Single-Chain Construct

1JBJ の概要

• エントリーDOI: 10.2210/pdb1jbj/pdb
• NMR情報: BMRB: 5072
• 分子名称: CD3 Epsilon and gamma Ectodomain Fragment Complex (1 entity in total)
• 機能のキーワード: beta-sheet, c2-set immunoglobulin superfamily, h-bonded g strand pair, single-chain, immune system
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P11942
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20632.84

構造登録者

Sun, Z.-Y.J.,Kim, K.S.,Wagner, G.,Reinherz, E.L. (登録日: 2001-06-05, 公開日: 2001-12-05, 最終更新日: 2024-11-13)

主引用文献

Sun, Z.J.,Kim, K.S.,Wagner, G.,Reinherz, E.L.
Mechanisms contributing to T cell receptor signaling and assembly revealed by the solution structure of an ectodomain fragment of the CD3 epsilon gamma heterodimer.
Cell(Cambridge,Mass.), 105:913-923, 2001

PubMed Abstract: The T cell receptor (TCR) consists of genetically diverse disulfide-linked alpha and beta chains in noncovalent association with the invariant CD3 subunits. CD3 epsilon and CD3 gamma are integral components of both the TCR and pre-TCR. Here, we present the solution structure of a heterodimeric CD3 epsilon gamma ectodomain complex. A unique side-to-side hydrophobic interface between the two C2-set immunoglobulin-like domains and parallel pairing of their respective C-terminal beta strands are revealed. Mutational analysis confirms the importance of the distinctive linkage as well as the membrane proximal stalk motif (RxCxxCxE) for domain-domain association. These biochemical and structural analyses offer insights into the modular pairwise association of CD3 invariant chains. More importantly, the findings suggest how the rigidified CD3 elements participate in TCR-based signal transduction.

PubMed: 11439187
DOI: 10.1016/S0092-8674(01)00395-6

実験手法

SOLUTION NMR