1JBA

UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND

1JBA の概要

• エントリーDOI: 10.2210/pdb1jba/pdb
• NMR情報: BMRB: 4492
• 分子名称: PROTEIN (GUANYLATE CYCLASE ACTIVATING PROTEIN 2), CALCIUM ION (2 entities in total)
• 機能のキーワード: ef-hand, calcium-binding protein, guanylyl cyclase regulation, lyase
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Cell membrane: P51177
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23875.08

構造登録者

Ames, J.B.,Dizhoor, A.M.,Ikura, M.,Palczewski, K.,Stryer, L. (登録日: 1999-04-03, 公開日: 1999-12-10, 最終更新日: 2023-12-27)

主引用文献

Ames, J.B.,Dizhoor, A.M.,Ikura, M.,Palczewski, K.,Stryer, L.
Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases.
J.Biol.Chem., 274:19329-19337, 1999

PubMed Abstract: Guanylyl cyclase activating protein-2 (GCAP-2) is a Ca2+-sensitive regulator of phototransduction in retinal photoreceptor cells. GCAP-2 activates retinal guanylyl cyclases at low Ca2+ concentration (<100 nM) and inhibits them at high Ca2+ (>500 nM). The light-induced lowering of the Ca2+ level from approximately 500 nM in the dark to approximately 50 nM following illumination is known to play a key role in visual recovery and adaptation. We report here the three-dimensional structure of unmyristoylated GCAP-2 with three bound Ca2+ ions as determined by nuclear magnetic resonance spectroscopy of recombinant, isotopically labeled protein. GCAP-2 contains four EF-hand motifs arranged in a compact tandem array like that seen previously in recoverin. The root mean square deviation of the main chain atoms in the EF-hand regions is 2.2 A in comparing the Ca2+-bound structures of GCAP-2 and recoverin. EF-1, as in recoverin, does not bind calcium because it contains a disabling Cys-Pro sequence. GCAP-2 differs from recoverin in that the calcium ion binds to EF-4 in addition to EF-2 and EF-3. A prominent exposed patch of hydrophobic residues formed by EF-1 and EF-2 (Leu24, Trp27, Phe31, Phe45, Phe48, Phe49, Tyr81, Val82, Leu85, and Leu89) may serve as a target-binding site for the transmission of calcium signals to guanylyl cyclase.

PubMed: 10383444
DOI: 10.1074/jbc.274.27.19329

実験手法

SOLUTION NMR