1JB5

CRYSTAL STRUCTURE OF NTF2 M118E MUTANT

1JB5 の概要

• エントリーDOI: 10.2210/pdb1jb5/pdb
• 関連するPDBエントリー: 1JB2 1JB4
• 分子名称: NUCLEAR TRANSPORT FACTOR 2 (2 entities in total)
• 機能のキーワード: ntf2, transport, protein transport
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cytoplasm (By similarity): P61972
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28950.64

構造登録者

Chaillan-Huntington, C.,Butler, P.J.,Huntington, J.A.,Akin, D.,Feldherr, C.,Stewart, M. (登録日: 2001-06-01, 公開日: 2002-03-13, 最終更新日: 2023-10-25)

主引用文献

Chaillan-Huntington, C.,Butler, P.J.,Huntington, J.A.,Akin, D.,Feldherr, C.,Stewart, M.
NTF2 monomer-dimer equilibrium.
J.Mol.Biol., 314:465-477, 2001

PubMed Abstract: Nuclear transport factor 2 (NTF2) mediates nuclear import of RanGDP, a central component of many nuclear trafficking pathways. NTF2 is a homodimer and each chain has independent binding sites for RanGDP and nuclear pore proteins (nucleoporins) that contain FxFG sequence repeats. We show here that the monomer-dimer dissociation constant for NTF2 obtained by sedimentation equilibrium ultracentrifugation is in the micromolar range, indicating that a substantial proportion of cellular NTF2 may be monomeric. To investigate the functional significance of NTF2 dimerization, we engineered a series of point mutations at the dimerization interface and one of these (M118E) remained monomeric below concentrations of 150 microM. CD spectra and X-ray crystallography showed that M118E-NTF2 preserved the wild-type NTF2 fold, although its thermal stability was 20 deg. C lower than that of the wild-type. M118E-NTF2 bound both RanGDP and FxFG nucleoporins less strongly, suggesting that dissociation of the NTF2 dimer could facilitate RanGDP release and thus nucleotide exchange after it had been transported into the nucleus. Moreover, colloidal gold coated with M118E-NTF2 showed reduced binding to Xenopus oocyte nuclear pores. Overall, our results indicate that dimer formation is important for NTF2 function and give insight into the formation of heterodimers by mRNA export factors such as TAP1 and NXT1 that contain NTF2-homology domains.

PubMed: 11846560
DOI: 10.1006/jmbi.2001.5136

実験手法

X-RAY DIFFRACTION (2.3 Å)