1J9I

STRUCTURE OF THE DNA BINDING DOMAIN OF THE GPNU1 SUBUNIT OF LAMBDA TERMINASE

1J9I の概要

• エントリーDOI: 10.2210/pdb1j9i/pdb
• NMR情報: BMRB: 4752
• 分子名称: TERMINASE SMALL SUBUNIT (1 entity in total)
• 機能のキーワード: dna binding domain, homodimer, viral assembly, lambda, winged helix-turn-helix, terminase, viral protein
• 由来する生物種: Enterobacteria phage lambda
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15637.54

構造登録者

De Beer, T.,Meyer, J.,Ortega, M.,Yang, Q.,Maes, L.,Duffy, C.,Berton, N.,Sippy, J.,Overduin, M.,Feiss, M.,Catalano, C. (登録日: 2001-05-25, 公開日: 2002-08-14, 最終更新日: 2024-05-22)

主引用文献

de Beer, T.,Fang, J.,Ortega, M.,Yang, Q.,Maes, L.,Duffy, C.,Berton, N.,Sippy, J.,Overduin, M.,Feiss, M.,Catalano, C.E.
Insights into specific DNA recognition during the assembly of a viral genome packaging machine.
Mol.Cell, 9:981-991, 2002

PubMed Abstract: Terminase enzymes mediate genome "packaging" during the reproduction of DNA viruses. In lambda, the gpNu1 subunit guides site-specific assembly of terminase onto DNA. The structure of the dimeric DNA binding domain of gpNu1 was solved using nuclear magnetic resonance spectroscopy. Its fold contains a unique winged helix-turn-helix (wHTH) motif within a novel scaffold. Surprisingly, a predicted P loop ATP binding motif is in fact the wing of the DNA binding motif. Structural and genetic analysis has identified determinants of DNA recognition specificity within the wHTH motif and the DNA recognition sequence. The structure reveals an unexpected DNA binding mode and provides a mechanistic basis for the concerted action of gpNu1 and Escherichia coli integration host factor during assembly of the packaging machinery.

PubMed: 12049735
DOI: 10.1016/S1097-2765(02)00537-3

実験手法

SOLUTION NMR