1J90

Crystal Structure of Drosophila Deoxyribonucleoside Kinase

1J90 の概要

• エントリーDOI: 10.2210/pdb1j90/pdb
• 分子名称: Deoxyribonucleoside kinase, SULFATE ION, 2'-DEOXYCYTIDINE, ... (4 entities in total)
• 機能のキーワード: protein-deoxynucleoside complex, transferase
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54459.97

構造登録者

Johansson, K.,Ramaswamy, S.,Ljungkrantz, C.,Knecht, W.,Piskur, J.,Munch-Petersen, B.,Eriksson, S.,Eklund, H. (登録日: 2001-05-23, 公開日: 2001-11-28, 最終更新日: 2024-02-07)

主引用文献

Johansson, K.,Ramaswamy, S.,Ljungcrantz, C.,Knecht, W.,Piskur, J.,Munch-Petersen, B.,Eriksson, S.,Eklund, H.
Structural basis for substrate specificities of cellular deoxyribonucleoside kinases.
Nat.Struct.Biol., 8:616-620, 2001

PubMed Abstract: Deoxyribonucleoside kinases phosphorylate deoxyribonucleosides and activate a number of medically important nucleoside analogs. Here we report the structure of the Drosophila deoxyribonucleoside kinase with deoxycytidine bound at the nucleoside binding site and that of the human deoxyguanosine kinase with ATP at the nucleoside substrate binding site. Compared to the human kinase, the Drosophila kinase has a wider substrate cleft, which may be responsible for the broad substrate specificity of this enzyme. The human deoxyguanosine kinase is highly specific for purine substrates; this is apparently due to the presence of Arg 118, which provides favorable hydrogen bonding interactions with the substrate. The two new structures provide an explanation for the substrate specificity of cellular deoxyribonucleoside kinases.

PubMed: 11427893
DOI: 10.1038/89661

実験手法

X-RAY DIFFRACTION (2.56 Å)