1J8G

X-ray Analysis of a RNA Tetraplex r(uggggu)4 at Ultra-High Resolution

1J8G の概要

• エントリーDOI: 10.2210/pdb1j8g/pdb
• 分子名称: 5'-R(*UP*GP*GP*GP*GP*U)-3', SPERMINE, STRONTIUM ION, ... (6 entities in total)
• 機能のキーワード: rna tetraplex, sr ions, u tetrad, octaplex, rna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 8862.30

構造登録者

Deng, J.,Xiong, Y.,Sundaralingam, M. (登録日: 2001-05-21, 公開日: 2001-11-23, 最終更新日: 2024-02-07)

主引用文献

Deng, J.,Xiong, Y.,Sundaralingam, M.
X-ray analysis of an RNA tetraplex (UGGGGU)(4) with divalent Sr(2+) ions at subatomic resolution (0.61 A).
Proc.Natl.Acad.Sci.USA, 98:13665-13670, 2001

PubMed Abstract: Four-stranded guanine tetraplexes in RNA have been identified to be involved in crucial biological functions, such as dimerization of retroviral RNA, translational repression, and mRNA turnover. However, the structural basis for these biological processes is still largely unknown. Here we report the RNA tetraplex structure (UGGGGU)(4) at ultra-high resolution (0.61 A). The space group is P42(1)2, and cell constants are a = b = 36.16 A and c = 74.09 A. The structure was solved by the multiple-wavelength anomalous dispersion method using a set of three-wavelength data of the isomorphous bromo derivative (br)UGGGGU and refined to 0.61-A resolution. Each of the four strands in the asymmetric unit forms a parallel tetraplex with symmetry-related molecules. The tetraplex molecules stack on one another in opposite polarity (head-to-head or tail-to-tail) to form a pseudocontinuous column. All of the 5'-end uridines rotate around the backbone of G2, swing out, and form unique octaplexes with the neighboring G tetraplexes, whereas the 3'-end uridines are stacked-in and form uridine tetrads. All of the bases are anti, and the riboses are in the mixed C2'- and C3'-puckering mode. Strontium ions are observed in every other guanine tetrad plane, sitting on the fourfold axis and associated to the eight O6 atoms of neighboring guanine bases in a bipyramidal-antiprism geometry. The hydrogens are clearly observed in the structure.

PubMed: 11707581
DOI: 10.1073/pnas.241374798

実験手法

X-RAY DIFFRACTION (0.61 Å)