1J7V

HUMAN IL-10 / IL-10R1 COMPLEX

Summary for 1J7V

• Entry DOI: 10.2210/pdb1j7v/pdb
• Descriptor: INTERLEUKIN-10, INTERLEUKIN-10 RECEPTOR ALPHA CHAIN (3 entities in total)
• Functional Keywords: cytokine receptor complex, 4 helix bundle, class 2 receptor, interleukin-10, cytokine-receptor complex, cytokine/receptor
• Biological source: Homo sapiens (human); More
• Cellular location: Secreted: P22301; Membrane; Single-pass type I membrane protein: Q13651
• Total number of polymer chains: 2
• Total formula weight: 43179.00

Authors

Josephson, K.,Logsdon, N.,Walter, M.R. (deposition date: 2001-05-18, release date: 2001-09-19, Last modification date: 2024-10-30)

Primary citation

Josephson, K.,Logsdon, N.J.,Walter, M.R.
Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site.
Immunity, 15:35-46, 2001

PubMed Abstract: Interleukin 10 (IL-10) is a dimeric cytokine that plays a central role in suppressing inflammatory responses. These activities are dependent on the interaction of IL-10 with its high-affinity receptor (IL-10R1). This intermediate complex must subsequently recruit the low-affinity IL-10R2 chain before cell signaling can occur. Here we report the 2.9 A crystal structure of IL-10 bound to a soluble form of IL-10R1 (sIL-10R1). The complex consists of two IL-10s and four sIL-10R1 molecules. Several residues in the IL-10/sIL-10R1 interface are conserved in all IL-10 homologs and their receptors. The data suggests that formation of the active IL-10 signaling complex occurs by a novel molecular recognition paradigm where IL-10R1 and IL-10R2 both recognize the same binding site on IL-10.

PubMed: 11485736
DOI: 10.1016/S1074-7613(01)00169-8

Experimental method

X-RAY DIFFRACTION (2.9 Å)