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1J7O

Solution structure of Calcium-calmodulin N-terminal domain

Summary for 1J7O
Entry DOI10.2210/pdb1j7o/pdb
Related1CLL 1EXR 1F70
DescriptorCalmodulin, CALCIUM ION (2 entities in total)
Functional Keywordsef hands, calcium binding, helix bundle, mini beta strand, metal binding protein
Biological sourceHomo sapiens (human)
Cellular locationCytoplasm, cytoskeleton, spindle: P62158
Total number of polymer chains1
Total formula weight8534.51
Authors
Chou, J.J.,Klee, C.B.,Bax, A. (deposition date: 2001-05-17, release date: 2001-11-07, Last modification date: 2024-05-22)
Primary citationChou, J.J.,Li, S.,Klee, C.B.,Bax, A.
Solution structure of Ca(2+)-calmodulin reveals flexible hand-like properties of its domains.
Nat.Struct.Biol., 8:990-997, 2001
Cited by
PubMed Abstract: The solution structure of Ca(2+)-ligated calmodulin is determined from residual dipolar couplings measured in a liquid crystalline medium and from a large number of heteronuclear J couplings for defining side chains. Although the C-terminal domain solution structure is similar to the X-ray crystal structure, the EF hands of the N-terminal domain are considerably less open. The substantial differences in interhelical angles correspond to negligible changes in short interproton distances and, therefore, cannot be identified by comparison of NOEs and X-ray data. NOE analysis, however, excludes a two-state equilibrium in which the closed apo conformation is partially populated in the Ca(2+)-ligated state. The difference between the crystal and solution structures of Ca(2+)-calmodulin indicates considerable backbone plasticity within the domains of calmodulin, which is key to their ability to bind a wide range of targets. In contrast, the vast majority of side chains making up the target binding surface are locked into the same chi(1) rotameric states as in complexes with target peptide.
PubMed: 11685248
DOI: 10.1038/nsb1101-990
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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