1J7O

Solution structure of Calcium-calmodulin N-terminal domain

1J7O の概要

• エントリーDOI: 10.2210/pdb1j7o/pdb
• 関連するPDBエントリー: 1CLL 1EXR 1F70
• 分子名称: Calmodulin, CALCIUM ION (2 entities in total)
• 機能のキーワード: ef hands, calcium binding, helix bundle, mini beta strand, metal binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytoskeleton, spindle: P62158
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8534.51

構造登録者

Chou, J.J.,Klee, C.B.,Bax, A. (登録日: 2001-05-17, 公開日: 2001-11-07, 最終更新日: 2024-05-22)

主引用文献

Chou, J.J.,Li, S.,Klee, C.B.,Bax, A.
Solution structure of Ca(2+)-calmodulin reveals flexible hand-like properties of its domains.
Nat.Struct.Biol., 8:990-997, 2001

PubMed Abstract: The solution structure of Ca(2+)-ligated calmodulin is determined from residual dipolar couplings measured in a liquid crystalline medium and from a large number of heteronuclear J couplings for defining side chains. Although the C-terminal domain solution structure is similar to the X-ray crystal structure, the EF hands of the N-terminal domain are considerably less open. The substantial differences in interhelical angles correspond to negligible changes in short interproton distances and, therefore, cannot be identified by comparison of NOEs and X-ray data. NOE analysis, however, excludes a two-state equilibrium in which the closed apo conformation is partially populated in the Ca(2+)-ligated state. The difference between the crystal and solution structures of Ca(2+)-calmodulin indicates considerable backbone plasticity within the domains of calmodulin, which is key to their ability to bind a wide range of targets. In contrast, the vast majority of side chains making up the target binding surface are locked into the same chi(1) rotameric states as in complexes with target peptide.

PubMed: 11685248
DOI: 10.1038/nsb1101-990

実験手法

SOLUTION NMR