1J6S

Crystal Structure of an RNA Tetraplex (UGAGGU)4 with A-tetrads, G-tetrads, U-tetrads and G-U octads

1J6S の概要

• エントリーDOI: 10.2210/pdb1j6s/pdb
• 分子名称: 5'-R(*(BRUP*GP*AP*GP*GP*U)-3', BARIUM ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: rna, tetraplex, g-u octads, a-tetrads, u-tetrads, g-tetrads
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 9262.91

構造登録者

Pan, B.,Xiong, Y.,Shi, K.,Deng, J.,Sundaralingam, M. (登録日: 2002-07-10, 公開日: 2003-08-05, 最終更新日: 2023-12-27)

主引用文献

Pan, B.,Xiong, Y.,Shi, K.,Deng, J.,Sundaralingam, M.
Crystal structure of an RNA purine-rich tetraplex containing adenine tetrads: implications for specific binding in RNA tetraplexes
Structure, 11:815-823, 2003

PubMed Abstract: Purine-rich regions in DNA and RNA may contain both guanines and adenines, which have various biological functions. Here we report the crystal structure of an RNA purine-rich fragment containing both guanine and adenine at 1.4 A resolution. Adenines form an adenine tetrad in the N6-H em leader N7 conformation. Substitution of an adenine tetrad in the guanine tetraplex does not change the global conformation but introduces irregularity in both the hydrogen bonding interaction pattern in the groove and the metal ion binding pattern in the central cavity of the tetraplex. The irregularity in groove binding may be critical for specific binding in tetraplexes. The formation of G-U octads provides a mechanism for interaction in the groove. Ba(2+) ions prefer to bind guanine tetrads, and adenine tetrads can only be bound by Na(+) ions, illustrating the binding selectivity of metal ions for the tetraplex.

PubMed: 12842044
DOI: 10.1016/S0969-2126(03)00107-2

実験手法

X-RAY DIFFRACTION (1.4 Å)