1J5H

Solution Structure of Apo-Neocarzinostatin

Summary for 1J5H

• Entry DOI: 10.2210/pdb1j5h/pdb
• Descriptor: Apo-Neocarzinostatin (1 entity in total)
• Functional Keywords: beta sandwich, igg fold, antibiotic
• Biological source: Streptomyces carzinostaticus
• Total number of polymer chains: 1
• Total formula weight: 12227.27

Authors

Urbaniak, M.D.,Muskett, F.W.,Finucane, M.D.,Caddick, S.,Woolfson, D.N. (deposition date: 2002-05-02, release date: 2002-09-11, Last modification date: 2024-11-06)

Primary citation

Urbaniak, M.D.,Muskett, F.W.,Finucane, M.D.,Caddick, S.,Woolfson, D.N.
Solution Structure of a Novel Chromoprotein Derived from Apo-Neocarzinostatin and a Synthetic Chromophore
Biochemistry, 41:11731-11739, 2002

PubMed Abstract: The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.

PubMed: 12269815
DOI: 10.1021/bi0262146

Experimental method

SOLUTION NMR