1J5H

Solution Structure of Apo-Neocarzinostatin

1J5H の概要

• エントリーDOI: 10.2210/pdb1j5h/pdb
• 分子名称: Apo-Neocarzinostatin (1 entity in total)
• 機能のキーワード: beta sandwich, igg fold, antibiotic
• 由来する生物種: Streptomyces carzinostaticus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12227.27

構造登録者

Urbaniak, M.D.,Muskett, F.W.,Finucane, M.D.,Caddick, S.,Woolfson, D.N. (登録日: 2002-05-02, 公開日: 2002-09-11, 最終更新日: 2024-11-06)

主引用文献

Urbaniak, M.D.,Muskett, F.W.,Finucane, M.D.,Caddick, S.,Woolfson, D.N.
Solution Structure of a Novel Chromoprotein Derived from Apo-Neocarzinostatin and a Synthetic Chromophore
Biochemistry, 41:11731-11739, 2002

PubMed Abstract: The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.

PubMed: 12269815
DOI: 10.1021/bi0262146

実験手法

SOLUTION NMR