1J5C

SOLUTION STRUCTURE OF OXIDIZED PARAMAGNETIC CU(II) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803

「1I0W」から置き換えられました

1J5C の概要

• エントリーDOI: 10.2210/pdb1j5c/pdb
• 関連するPDBエントリー: 1J5D
• 分子名称: PLASTOCYANIN, COPPER (II) ION (2 entities in total)
• 機能のキーワード: copper protein beta barrel electron transfer, electron transport
• 由来する生物種: Synechocystis sp. PCC 6803
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10312.98

構造登録者

Bertini, I.,Ciurli, S.,Dikiy, A.,Fernandez, C.O.,Luchinat, C.,Safarov, N.,Shumilin, S.,Vila, A.J. (登録日: 2002-04-02, 公開日: 2002-04-10, 最終更新日: 2023-12-27)

主引用文献

Bertini, I.,Ciurli, S.,Dikiy, A.,Fernandez, C.O.,Luchinat, C.,Safarov, N.,Shumilin, S.,Vila, A.J.
The first solution structure of a paramagnetic copper(II) protein: the case of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803.
J.Am.Chem.Soc., 123:2405-2413, 2001

PubMed Abstract: The NMR solution structure of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803 is here reported. The protein contains paramagnetic copper(II), whose electronic relaxation times are quite unfavorable for NMR solution studies. The structure has been solved on the basis of 1041 meaningful NOESY cross-peaks, 18 1D NOEs, 26 T(1) values, 96 dihedral angle constraints, and 18 H-bonds. The detection of broad hyperfine-shifted signals and their full assignment allowed the identification of the copper(II) ligands and the determination of the Cu-S-C-H dihedral angle for the coordinated cysteine. The global root-mean-square deviation from the mean structure for the solution structure family is 0.72 +/- 0.14 and 1.16 +/- 0.17 A for backbone and heavy atoms, respectively. The structure is overall quite satisfactory and represents a breakthrough, in that it includes paramagnetic copper proteins among the metalloproteins for which solution structures can be afforded. The comparison with the available X-ray structure of a triple mutant is also performed.

PubMed: 11456890
DOI: 10.1021/ja0033685

実験手法

SOLUTION NMR