1J55

The Crystal Structure of Ca+-bound Human S100P Determined at 2.0A Resolution by X-ray

1J55 の概要

• エントリーDOI: 10.2210/pdb1j55/pdb
• 分子名称: S-100P PROTEIN, CALCIUM ION (3 entities in total)
• 機能のキーワード: metal binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P25815
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10492.04

構造登録者

Zhang, H.,Wang, G.,Ding, Y.,Wang, Z.,Barraclough, R.,Rudland, P.S.,Fernig, D.G.,Rao, Z. (登録日: 2002-01-25, 公開日: 2003-01-07, 最終更新日: 2023-12-27)

主引用文献

Zhang, H.,Wang, G.,Ding, Y.,Wang, Z.,Barraclough, R.,Rudland, P.S.,Fernig, D.G.,Rao, Z.
The Crystal Structure at 2A Resolution of the Ca2+-binding Protein S100P
J.Mol.Biol., 325:785-794, 2003

PubMed Abstract: S100P is a small calcium-binding protein of the S100 EF-hand-containing family of proteins. Elevated levels of its mRNA are reported to be associated with the progression to hormone independence and metastasis of prostate cancer and to be associated with loss of senescence in human breast epithelial cells in vitro. The first structure of human recombinant S100P in calcium-bound form is now reported at 2.0A resolution by X-ray diffraction. A flexible linker connects the two EF-hand motifs. The protein exists as a homodimer formed by non-covalent interactions between large hydrophobic areas on monomeric S100P. Experiments with an optical biosensor to study binding parameters of the S100P monomer interaction showed that the association rate constant was faster in the presence of calcium than in their absence, whereas the dissociation rate constant was independent of calcium. The K(d) values were 64(+/-24)nM and 2.5(+/-0.8) microM in the presence and in the absence of calcium ions, respectively. Dimerization of S100P is demonstrated in vivo using the yeast two-hybrid system. The effect of mutation of specific amino acids suggests that dimerization in vivo can be affected by amino acids on the dimer interface and in the hydrophobic core.

PubMed: 12507480
DOI: 10.1016/S0022-2836(02)01278-0

実験手法

X-RAY DIFFRACTION (2 Å)