1J4M

Minimized average structure of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)

1J4M の概要

• エントリーDOI: 10.2210/pdb1j4m/pdb
• 関連するPDBエントリー: 1K43
• 分子名称: MBH12 (1 entity in total)
• 機能のキーワード: beta-hairpin, de novo protein
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1703.88

構造登録者

Pastor, M.T.,Lopez de la Paz, M.,Lacroix, E.,Serrano, L.,Perez-Paya, E. (登録日: 2001-10-10, 公開日: 2001-10-17, 最終更新日: 2023-12-27)

主引用文献

Pastor, M.T.,Lopez de la Paz, M.,Lacroix, E.,Serrano, L.,Perez-Paya, E.
Combinatorial approaches: a new tool to search for highly structured beta-hairpin peptides.
Proc.Natl.Acad.Sci.USA, 99:614-619, 2002

PubMed Abstract: Here we present a combinatorial approach to evolve a stable beta-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a beta-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20(4) sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-aa-long peptides that in plain buffered solutions showed a percentage of beta-hairpin structure higher than 70%. Our results show how combinatorial approaches can be used to obtain highly structured peptide sequences that could be used as templates in which functionality can be introduced.

PubMed: 11782528
DOI: 10.1073/pnas.012583999

実験手法

SOLUTION NMR