1J2M

Solution structure of CPI-17(22-120)

1J2M の概要

• エントリーDOI: 10.2210/pdb1j2m/pdb
• 関連するPDBエントリー: 1J2N
• 分子名称: 17-kDa PKC-potentiated inhibitory protein of PP1 (1 entity in total)
• 機能のキーワード: helix bundle, protein binding
• 由来する生物種: Sus scrofa (pig)
• 細胞内の位置: Cytoplasm: O18734
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11546.08

構造登録者

Ohki, S.,Eto, M.,Takada, R.,Shimizu, M.,Brautigan, D.L.,Kainosho, M. (登録日: 2003-01-07, 公開日: 2003-06-17, 最終更新日: 2023-12-27)

主引用文献

Ohki, S.,Eto, M.,Shimizu, M.,Takada, R.,Brautigan, D.L.,Kainosho, M.
Distinctive Solution Conformation of Phosphatase Inhibitor CPI-17 Substituted with Aspartate at the Phosphorylation-site Threonine Residue
J.Mol.Biol., 326:1539-1547, 2003

PubMed Abstract: We present solution NMR structures for wild-type and mutated forms of CPI-17, a phosphoinhibitor for protein phosphatase 1. Phosphorylation of Thr38 of CPI-17 produces a >1000-fold increase in inhibitory potency for myosin phosphatase. We compared the 1H-15N heteronuclear single quantum coherence spectroscopy (HSQC) chemical shifts of wild-type CPI-17, partially phosphorylated CPI-17 and CPI-17 with Thr38 replaced with Asp to introduce a negative charge. There was a switch in the protein conformation due to either Asp substitution or phosphorylation, so we determined the solution NMR structure of the CPI-17 T38D mutant as a model for the active (phospho-) conformation. The structures reveal a molecular switch in conformation that involves the rotation of two of the four helices in the four helix bundle. Despite this conformational switch, there was little increase in the inhibitory potency with T38D. We propose that for this inhibitor, a negative charge at residue 38 is sufficient to trigger an active conformation, but a phosphoryl group is required for full inhibitory potency against protein phosphatase-1.

PubMed: 12595264
DOI: 10.1016/S0022-2836(03)00048-2

実験手法

SOLUTION NMR