1J23

Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain

1J23 の概要

• エントリーDOI: 10.2210/pdb1j23/pdb
• 関連するPDBエントリー: 1J22 1J24 1J25
• 分子名称: ATP-dependent RNA helicase, putative (2 entities in total)
• 機能のキーワード: structure-specific endonuclease, hydrolase
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16116.66

構造登録者

Nishino, T.,Komori, K.,Ishino, Y.,Morikawa, K. (登録日: 2002-12-25, 公開日: 2003-04-22, 最終更新日: 2024-04-03)

主引用文献

Nishino, T.,Komori, K.,Ishino, Y.,Morikawa, K.
X-Ray and Biochemical Anatomy of an Archaeal XPF/Rad1/Mus81 Family Nuclease. Similarity between Its Endonuclease Domain and Restriction Enzymes
Structure, 11:445-457, 2003

PubMed Abstract: The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. Here, we report the domain organization of an archaeal homolog (Hef) of this family and the X-ray crystal structure of the middle domain, with the nuclease activity. The nuclease domain architecture exhibits remarkable similarity to those of restriction endonucleases, including the correspondence of the GDX(n)ERKX(3)D signature motif in Hef to the PDX(n)(E/D)XK motif in restriction enzymes. This structural study also suggests that the XPF/Rad1/Mus81/ERCC1 proteins form a dimer through each interface of the nuclease domain and the helix-hairpin-helix domain. Simultaneous disruptions of both interfaces result in their dissociation into separate monomers, with strikingly reduced endonuclease activities.

PubMed: 12679022
DOI: 10.1016/S0969-2126(03)00046-7

実験手法

X-RAY DIFFRACTION (1.78 Å)