1J1H

Solution structure of a tmRNA-binding protein, SmpB, from Thermus thermophilus

1J1H の概要

• エントリーDOI: 10.2210/pdb1j1h/pdb
• 分子名称: Small Protein B (1 entity in total)
• 機能のキーワード: smpb, ssra associated protein, ob fold, riken structural genomics/proteomics initiative, rsgi, structural genomics, rna binding protein
• 由来する生物種: Thermus thermophilus
• 細胞内の位置: Cytoplasm (Potential): Q8RR57
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16785.49

構造登録者

Someya, T.,Nameki, N.,Hosoi, H.,Suzuki, S.,Hatanaka, H.,Fujii, M.,Terada, T.,Shirouzu, M.,Inoue, Y.,Shibata, T.,Kuramitsu, S.,Yokoyama, S.,Kawai, G.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2002-12-04, 公開日: 2003-02-11, 最終更新日: 2023-12-27)

主引用文献

Someya, T.,Nameki, N.,Hosoi, H.,Suzuki, S.,Hatanaka, H.,Fujii, M.,Terada, T.,Shirouzu, M.,Inoue, Y.,Shibata, T.,Kuramitsu, S.,Yokoyama, S.,Kawai, G.
Solution structure of a tmRNA-binding protein, SmpB, from Thermus thermophilus
FEBS Lett., 535:94-100, 2003

PubMed Abstract: Small protein B (SmpB) is required for trans-translation, binding specifically to tmRNA. We show here the solution structure of SmpB from an extremely thermophilic bacterium, Thermus thermophilus HB8, determined by heteronuclear nuclear magnetic resonance methods. The core of the protein consists of an antiparallel beta-barrel twisted up from eight beta-strands, each end of which is capped with the second or third helix, and the first helix is located beside the barrel. Its C-terminal sequence (20 residues), which is rich in basic residues, shows a poorly structured form, as often seen in isolated ribosomal proteins. The results are discussed in relation to the oligonucleotide binding fold.

PubMed: 12560085
DOI: 10.1016/S0014-5793(02)03880-2

実験手法

SOLUTION NMR