1J10

beta-amylase from Bacillus cereus var. mycoides in complex with GGX

1J10 の概要

• エントリーDOI: 10.2210/pdb1j10/pdb
• 関連するPDBエントリー: 1J0Y 1J0Z 1J11 1J12 5BCA
• 分子名称: Beta-amylase, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-xylopyranose, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-xylopyranose, ... (5 entities in total)
• 機能のキーワード: hydrolase, beta-amylase, raw-starch binding domain
• 由来する生物種: Bacillus cereus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 237389.70

構造登録者

Oyama, T.,Miyake, H.,Kusunoki, M.,Nitta, Y. (登録日: 2002-11-25, 公開日: 2003-06-17, 最終更新日: 2024-11-13)

主引用文献

Oyama, T.,Miyake, H.,Kusunoki, M.,Nitta, Y.
Crystal Structures of beta-Amylase from Bacillus cereus var. mycoides in Complexes with Substrate Analogs and Affinity-Labeling Reagents
J.BIOCHEM.(TOKYO), 133:467-474, 2003

PubMed Abstract: The crystal structures of beta-amylase from Bacillus cereus var. mycoides in complexes with five inhibitors were solved. The inhibitors used were three substrate analogs, i.e. glucose, maltose (product), and a synthesized compound, O-alpha-D-glucopyranosyl-(1-->4)-O-alpha-D-glucopyranosyl-(1-->4)-D-xylopyranose (GGX), and two affinity-labeling reagents with an epoxy alkyl group at the reducing end of glucose. For all inhibitors, one molecule was bound at the active site cleft and the non-reducing end glucose of the four inhibitors except GGX was located at subsite 1, accompanied by a large conformational change of the flexible loop (residues 93-97), which covered the bound inhibitor. In addition, another molecule of maltose or GGX was bound about 30 A away from the active site. A large movement of residues 330 and 331 around subsite 3 was also observed upon the binding of GGX at subsites 3 to 5. Two affinity-labeling reagents, alpha-EPG and alpha-EBG, were covalently bound to a catalytic residue (Glu-172). A substrate recognition mechanism for the beta-amylase was discussed based on the modes of binding of these inhibitors in the active site cleft.

PubMed: 12761294
DOI: 10.1093/jb/mvg061

実験手法

X-RAY DIFFRACTION (2.1 Å)