1IXZ

Crystal structure of the FtsH ATPase domain from Thermus thermophilus

1IXZ の概要

• エントリーDOI: 10.2210/pdb1ixz/pdb
• 関連するPDBエントリー: 1IY0 1IY1 1IY2
• 分子名称: ATP-dependent metalloprotease FtsH, MERCURY (II) ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: aaa domain fold, hydrolase
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28074.83

構造登録者

Niwa, H.,Tsuchiya, D.,Makyio, H.,Yoshida, M.,Morikawa, K. (登録日: 2002-07-10, 公開日: 2002-11-06, 最終更新日: 2023-12-27)

主引用文献

Niwa, H.,Tsuchiya, D.,Makyio, H.,Yoshida, M.,Morikawa, K.
Hexameric ring structure of the ATPase domain of the membrane-integrated metalloprotease FtsH from Thermus thermophilus HB8
Structure, 10:1415-1423, 2002

PubMed Abstract: FtsH is a cytoplasmic membrane-integrated, ATP-dependent metalloprotease, which processively degrades both cytoplasmic and membrane proteins in concert with unfolding. The FtsH protein is divided into the N-terminal transmembrane region and the larger C-terminal cytoplasmic region, which consists of an ATPase domain and a protease domain. We have determined the crystal structures of the Thermus thermophilus FtsH ATPase domain in the nucleotide-free and AMP-PNP- and ADP-bound states, in addition to the domain with the extra preceding segment. Combined with the mapping of the putative substrate binding region, these structures suggest that FtsH internally forms a hexameric ring structure, in which ATP binding could cause a conformational change to facilitate transport of substrates into the protease domain through the central pore.

PubMed: 12377127
DOI: 10.1016/S0969-2126(02)00855-9

実験手法

X-RAY DIFFRACTION (2.2 Å)