1IVM

Solution structure of mouse lysozyme M

1IVM の概要

• エントリーDOI: 10.2210/pdb1ivm/pdb
• NMR情報: BMRB: 4751
• 分子名称: lysozyme M (1 entity in total)
• 機能のキーワード: hydrolase, glycosidase
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Secreted: P08905
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14839.64

構造登録者

Ueda, T.,Obita, T.,Imoto, T. (登録日: 2002-03-27, 公開日: 2002-05-08, 最終更新日: 2024-10-30)

主引用文献

Obita, T.,Ueda, T.,Imoto, T.
Solution structure and activity of mouse lysozyme M
CELL.MOL.LIFE SCI., 60:176-184, 2003

PubMed Abstract: The three-dimensional structure of mouse lysozyme M, glycoside hydrolase, with 130 amino acids has been determined by heteronuclear NMR spectroscopy. We found that mouse lysozyme M had four alpha-helices, two 3(10)helices, and a double- and a triple-stranded anti-parallel beta-sheet, and its structure was very similar to that of hen lysozyme in solution and in the crystalline state. The pH activity profile of p-nitrophenyl penta N-acetyl-beta-D-chitopentaoside hydrolysis by mouse lysozyme M was similar to that of hen lysozyme, but the hydrolytic activity of mouse lysozyme M was lower. From analyses of binding affinities of lysozymes to a substrate analogue and internal motions of lysozymes, we suggest that the lower activity of mouse lysozyme M was due to the larger dissociation constant of its enzyme-substrate complex and the restricted internal backbone motions in the molecule.

PubMed: 12613666
DOI: 10.1007/s000180300012

実験手法

SOLUTION NMR