1IU0

The first PDZ domain of PSD-95

1IU0 の概要

• エントリーDOI: 10.2210/pdb1iu0/pdb
• 関連するPDBエントリー: 1IU2
• 分子名称: PSD-95 (1 entity in total)
• 機能のキーワード: psd-95, pdz domain, post synaptic density, neuropeptide
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cell membrane; Peripheral membrane protein: P31016
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9839.04

構造登録者

Long, J.-F.,Tochio, H.,Wang, P.,Sala, C.,Niethammer, M.,Sheng, M.,Zhang, M. (登録日: 2002-02-18, 公開日: 2003-03-11, 最終更新日: 2023-12-27)

主引用文献

Long, J.-F.,Tochio, H.,Wang, P.,Fan, J.-S.,Sala, C.,Niethammer, M.,Sheng, M.,Zhang, M.
Supramodular structure and synergistic target binding of the N-terminal tandem PDZ domains of PSD-95
J.MOL.BIOL., 327:203-214, 2003

PubMed Abstract: PDZ domain proteins play critical roles in binding, clustering and subcellular targeting of membrane receptors and ion channels. PDZ domains in multi-PDZ proteins often are arranged in groups with highly conserved spacing and intervening sequences; however, the functional significance of such tandem arrangements of PDZs is unclear. We have solved the three-dimensional structure of the first two PDZ domains of postsynaptic density protein-95 (PSD-95 PDZ1 and PDZ2), which are closely linked to each other in the PSD-95 family of scaffold proteins. The two PDZs have limited freedom of rotation and their C-terminal peptide-binding grooves are aligned with each other with an orientation preference for binding to pairs of C termini extending in the same direction. Increasing the spacing between PDZ1 and PDZ2 resulted in decreased binding between PDZ12 and its dimeric targets. The same mutation impaired the functional ability of PSD-95 to cluster Kv1.4 potassium channels in heterologous cells. The data presented provide a molecular basis for preferential binding of PSD-95 to multimeric membrane proteins with appropriate C-terminal sequences.

PubMed: 12614619
DOI: 10.1016/S0022-2836(03)00113-X

実験手法

SOLUTION NMR