1ITM

ANALYSIS OF THE SOLUTION STRUCTURE OF HUMAN INTERLEUKIN 4 DETERMINED BY HETERONUCLEAR THREE-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE TECHNIQUES

1ITM の概要

• エントリーDOI: 10.2210/pdb1itm/pdb
• 分子名称: INTERLEUKIN-4 (1 entity in total)
• 機能のキーワード: cytokine
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P05112
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15120.44

構造登録者

Redfield, C.,Smith, L.J.,Boyd, J.,Lawrence, G.M.P.,Edwards, R.G.,Gershater, C.J.,Smith, R.A.G.,Dobson, C.M. (登録日: 1994-02-28, 公開日: 1994-05-31, 最終更新日: 2022-02-23)

主引用文献

Redfield, C.,Smith, L.J.,Boyd, J.,Lawrence, G.M.,Edwards, R.G.,Gershater, C.J.,Smith, R.A.,Dobson, C.M.
Analysis of the solution structure of human interleukin-4 determined by heteronuclear three-dimensional nuclear magnetic resonance techniques.
J.Mol.Biol., 238:23-41, 1994

PubMed Abstract: Human interleukin-4 (IL-4) is a member of the family of haemopoietic cytokines that modulate cell proliferation and differentiation within the immune system. It has a four-helix-bundle structure, and possesses a high degree of mobility in certain regions, notably in the two long loops running the length of the bundle in its up-up-down-down topology. Information from a variety of three-dimensional heteronuclear NMR experiments, including chemical shifts, coupling constants and NOE data, is analysed in terms of the solution structure of IL-4. In addition, structure calculations with and without specific restraints such as hydrogen bond location or torsion angle restrictions are compared in the light of the dynamic behaviour of the polypeptide chain. Particular emphasis is placed on defining the lengths and positions of secondary structure elements, and on the likely structural preferences within the less well ordered loop regions. The overall topology of IL-4 is compared with those defined in recent structure determinations of related proteins. This analysis is combined with recent mutagenesis data to propose a possible mode of interaction of IL-4 with its receptor.

PubMed: 8145254
DOI: 10.1006/jmbi.1994.1265

実験手法

SOLUTION NMR