1IRH

The Solution Structure of The Third Kunitz Domain of Tissue Factor Pathway Inhibitor

1IRH の概要

• エントリーDOI: 10.2210/pdb1irh/pdb
• 分子名称: tissue factor pathway inhibitor (1 entity in total)
• 機能のキーワード: non-protease inhibitor, kunitz domain, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P10646
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6921.82

構造登録者

Mine, S.,Yamazaki, T.,Miyata, T.,Hara, S.,Kato, H. (登録日: 2001-10-02, 公開日: 2002-02-06, 最終更新日: 2024-10-16)

主引用文献

Mine, S.,Yamazaki, T.,Miyata, T.,Hara, S.,Kato, H.
Structural mechanism for heparin-binding of the third Kunitz domain of human tissue factor pathway inhibitor.
Biochemistry, 41:78-85, 2002

PubMed Abstract: Tissue factor pathway inhibitor (TFPI) inhibits the activity of coagulation factor VIIa and Xa through its K1 and K2 domain, respectively, and the inhibitory activity is enhanced by heparin. The function of the K3 domain of TFPI has not been established, but the domain probably harbors a heparin binding site (HBS-2). We determined the three-dimensional solution structure of the TFPI K3 domain (Glu182-Gly242) by heteronuclear multidimensional NMR. The results showed that the molecule is composed of one antiparallel beta-sheet and one alpha-helix, and in overall structure is very similar to the K2 domain, with the rms deviation of 1.55 A for the 58 defined C(alpha) positions. However, the surface electrostatic properties of both domains are different each other. The lack of inhibitory activity of the K3 domain is explained by the absence of electrostatic interaction with factor Xa over a large surface area. A titration experiment with size-fractionated heparin showed that a heparin binding site was located in the vicinity of the alpha-helix. In this region, a positively charged cluster is formed by Lys213, Lys232, and Lys240, and the negatively charged sulfate groups of heparin bind there. The enhancement of inhibitory activity by heparin probably was not due to a conformational change to TFPI itself. It is likely that heparin simply increases the local concentration of TFPI on the cell surface and stabilizes the initial complex that forms.

PubMed: 11772005
DOI: 10.1021/bi011299g

実験手法

SOLUTION NMR