1IQP

Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus

1IQP の概要

• エントリーDOI: 10.2210/pdb1iqp/pdb
• 分子名称: RFCS, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: clamp loader, extended aaa-atpase domain, complex with adp, replication
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 226459.99

構造登録者

Oyama, T.,Ishino, Y.,Cann, I.K.O.,Ishino, S.,Morikawa, K. (登録日: 2001-07-24, 公開日: 2001-09-19, 最終更新日: 2023-12-27)

主引用文献

Oyama, T.,Ishino, Y.,Cann, I.K.O.,Ishino, S.,Morikawa, K.
Atomic Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus
Mol.Cell, 8:455-463, 2001

PubMed Abstract: In eukaryotic DNA replication, replication factor-C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands at replication forks. The eukaryotic RFC is a complex consisting of one large and four small subunits. We have determined the crystal structure of the clamp loader small subunit (RFCS) from Pyrococcus furiosus. The six subunits, of which four bind ADP in their canonical nucleotide binding clefts, assemble into a dimer of semicircular trimers. The crescent-like architecture of each subunit formed by the three domains resembles that of the delta' subunit of the E. coli clamp loader. The trimeric architecture of archaeal RFCS, with its mobile N-terminal domains, involves intersubunit interactions that may be conserved in eukaryotic functional complexes.

PubMed: 11545747
DOI: 10.1016/S1097-2765(01)00328-8

実験手法

X-RAY DIFFRACTION (2.8 Å)