1IJC

Solution Structure of Bucandin, a Neurotoxin from the Venom of the Malayan Krait

Summary for 1IJC

• Entry DOI: 10.2210/pdb1ijc/pdb
• Related: 1f94
• NMR Information: BMRB: 5097
• Descriptor: bucandin (1 entity in total)
• Functional Keywords: three-finger motif, two antiparallel beta-sheets, two and four stranded beta-sheets, toxin
• Biological source: Bungarus candidus
• Cellular location: Secreted: P81782
• Total number of polymer chains: 1
• Total formula weight: 7293.41

Authors

Torres, A.M.,Kini, R.M.,Nirthanan, S.,Kuchel, P.W. (deposition date: 2001-04-25, release date: 2001-12-21, Last modification date: 2024-10-16)

Primary citation

Torres, A.M.,Kini, R.M.,Selvanayagam, N.,Kuchel, P.W.
NMR structure of bucandin, a neurotoxin from the venom of the Malayan krait (Bungarus candidus).
Biochem.J., 360:539-548, 2001

PubMed Abstract: A high-resolution solution structure of bucandin, a neurotoxin from Malayan krait (Bungarus candidus), was determined by (1)H-NMR spectroscopy and molecular dynamics. The average backbone root-mean-square deviation for the 20 calculated structures and the mean structure is 0.47 A (1 A=0.1 nm) for all residues and 0.24 A for the well-defined region that spans residues 23-58. Secondary-structural elements include two antiparallel beta-sheets characterized by two and four strands. According to recent X-ray analysis, bucandin adopts a typical three-finger loop motif and yet it has some peculiar characteristics that set it apart from other common alpha-neurotoxins. The presence of a fourth strand in the second antiparallel beta-sheet had not been observed before in three-finger toxins, and this feature was well represented in the NMR structure. Although the overall fold of the NMR structure is similar to that of the X-ray crystal structure, there are significant differences between the two structures that have implications for the pharmacological action of the toxin. These include the extent of the beta-sheets, the conformation of the region spanning residues 42-49 and the orientation of some side chains. In comparison with the X-ray structure, the NMR structure shows that the hydrophobic side chains of Trp(27) and Trp(36) are stacked together and are orientated towards the tip of the middle loop. The NMR study also showed that the two-stranded beta-sheet incorporated in the first loop, as defined by residues 1-22, and the C-terminus from Asn(59), is probably flexible relative to the rest of the molecule. On the basis of the dispositions of the hydrophobic and hydrophilic side chains, the structure of bucandin is clearly different from those of cytotoxins.

PubMed: 11736642
DOI: 10.1042/0264-6021:3600539

Experimental method

SOLUTION NMR