1IIZ

Crystal Structure of the Induced Antibacterial Protein from Tasar Silkworm, Antheraea mylitta

1IIZ の概要

• エントリーDOI: 10.2210/pdb1iiz/pdb
• 分子名称: LYSOZYME (2 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Antheraea mylitta
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13761.42

構造登録者

Jain, D.,Nair, D.T.,Swaminathan, G.J.,Abraham, E.G.,Nagaraju, J.,Salunke, D.M. (登録日: 2001-04-24, 公開日: 2001-12-12, 最終更新日: 2024-10-30)

主引用文献

Jain, D.,Nair, D.T.,Swaminathan, G.J.,Abraham, E.G.,Nagaraju, J.,Salunke, D.M.
Structure of the induced antibacterial protein from tasar silkworm, Antheraea mylitta. Implications to molecular evolution.
J.Biol.Chem., 276:41377-41382, 2001

PubMed Abstract: The crystal structure of an antibacterial protein of immune origin (TSWAB), purified from tasar silkworm (Antheraea mylitta) larvae after induction by Escherichia coli infection, has been determined. This is the first insect lysozyme structure and represents induced lysozymes of innate immunity. The core structure of TSWAB is similar to c-type lysozymes and alpha-lactalbumins. However, TSWAB shows significant differences with respect to the other two proteins in the exposed loop regions. The catalytic residues in TSWAB are conserved with respect to the chicken lysozyme, indicating a common mechanism of action. However, differences in the noncatalytic residues in the substrate binding groove imply subtle differences in the specificity and the level of activity. Thus, conformational differences between TSWAB and chicken lysozyme exist, whereas functional mechanisms appear to be similar. On the other hand, alpha-lactalbumins and c-type lysozymes exhibit drastically different functions with conserved molecular conformation. It is evident that a common molecular scaffold is exploited in the three enzymes for apparently different physiological roles. It can be inferred on the basis of the structure-function comparison of these three proteins having common phylogenetic origin that the conformational changes in a protein are minimal during rapid evolution as compared with those in the normal course of evolution.

PubMed: 11522783
DOI: 10.1074/jbc.M104674200

実験手法

X-RAY DIFFRACTION (2.4 Å)