1IHW

SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, 40 STRUCTURES

1IHW の概要

• エントリーDOI: 10.2210/pdb1ihw/pdb
• 分子名称: HIV-1 INTEGRASE (1 entity in total)
• 機能のキーワード: dna-binding protein, aids, polyprotein, dna binding protein
• 由来する生物種: Human immunodeficiency virus 1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12278.37

構造登録者

Clore, G.M.,Lodi, P.J.,Ernst, J.A.,Gronenborn, A.M. (登録日: 1995-05-12, 公開日: 1996-07-11, 最終更新日: 2024-05-22)

主引用文献

Lodi, P.J.,Ernst, J.A.,Kuszewski, J.,Hickman, A.B.,Engelman, A.,Craigie, R.,Clore, G.M.,Gronenborn, A.M.
Solution structure of the DNA binding domain of HIV-1 integrase.
Biochemistry, 34:9826-9833, 1995

PubMed Abstract: The solution structure of the DNA binding domain of HIV-1 integrase (residues 220-270) has been determined by multidimensional NMR spectroscopy. The protein is a dimer in solution, and each subunit is composed of a five-stranded beta-barrel with a topology very similar to that of the SH3 domain. The dimer is formed by a stacked beta-interface comprising strands 2, 3, and 4, with the two triple-stranded antiparallel beta-sheets, one from each subunit, oriented antiparallel to each other. One surface of the dimer, bounded by the loop between strands beta 1 and beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x 23 x 12 A in cross section. Lys264, which has been shown from mutational data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.

PubMed: 7632683
DOI: 10.1021/bi00031a002

実験手法

SOLUTION NMR