1IF0

PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)

1IF0 の概要

• エントリーDOI: 10.2210/pdb1if0/pdb
• 関連するPDBエントリー: 1FH6
• 分子名称: PROTEIN (MAJOR CAPSID PROTEIN GP5) (1 entity in total)
• 機能のキーワード: bacteriophage, virus, capsid, cryoem, pseudo-atomic model., icosahedral virus
• 由来する生物種: Enterobacteria phage HK97
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 197958.25

構造登録者

Conway, J.F.,Wikoff, W.R.,Cheng, N.,Duda, R.L.,Hendrix, R.W.,Johnson, J.E.,Steven, A.C. (登録日: 2001-04-11, 公開日: 2001-05-02, 最終更新日: 2024-02-07)

主引用文献

Conway, J.F.,Wikoff, W.R.,Cheng, N.,Duda, R.L.,Hendrix, R.W.,Johnson, J.E.,Steven, A.C.
Virus maturation involving large subunit rotations and local refolding.
Science, 292:744-748, 2001

PubMed Abstract: Large-scale conformational changes transform viral precursors into infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked topology. We have visualized its precursor, Prohead-II, by cryoelectron microscopy and modeled the conformational change by appropriately adapting Head-II. Rigid-body rotations ( approximately 40 degrees) cause switching to an entirely different set of interactions; in addition, two motifs undergo refolding. These changes stabilize the capsid by increasing the surface area buried at interfaces and bringing the cross-link-forming residues, initially approximately 40 angstroms apart, close together. The inner surface of Prohead-II is negatively charged, suggesting that the transition is triggered electrostatically by DNA packaging.

PubMed: 11326105
DOI: 10.1126/science.1058069

実験手法

ELECTRON MICROSCOPY (12 Å)