1IE5

NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.

1IE5 の概要

• エントリーDOI: 10.2210/pdb1ie5/pdb
• NMR情報: BMRB: 5044
• 分子名称: NEURAL CELL ADHESION MOLECULE (1 entity in total)
• 機能のキーワード: intermediate immunoglobulin fold, cell adhesion
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Cell membrane; Single-pass membrane protein (Potential): P13590
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11851.09

構造登録者

Atkins, A.R.,Chung, J.,Deechongkit, S.,Little, E.B.,Edelman, G.M.,Wright, P.E.,Cunningham, B.A.,Dyson, H.J. (登録日: 2001-04-06, 公開日: 2001-08-08, 最終更新日: 2024-11-13)

主引用文献

Atkins, A.R.,Chung, J.,Deechongkit, S.,Little, E.B.,Edelman, G.M.,Wright, P.E.,Cunningham, B.A.,Dyson, H.J.
Solution structure of the third immunoglobulin domain of the neural cell adhesion molecule N-CAM: can solution studies define the mechanism of homophilic binding?
J.Mol.Biol., 311:161-172, 2001

PubMed Abstract: Homophilic binding of the neural cell adhesion molecule (N-CAM) mediates the calcium-independent cell-cell adhesion that is involved in neuronal development. Two hypotheses have been advanced for the mechanism of homophilic binding. Cell-based experiments have implicated each of the five extracellular immunoglobulin (Ig) domains of N-CAM in the homophilic adhesion interaction, and have predicted that the third domain (Ig III) self-associates. The alternative hypothesis is based on solution observations, which implicate a specific antiparallel interaction between the first two Ig domains (Ig I and Ig II). In order to test these hypotheses, we have determined a high-resolution solution structure of recombinant Ig III (sequence derived from chicken N-CAM) and examined the aggregation behavior of isolated Ig domains in solution. The structure shows that Ig III adopts a canonical Ig fold, in which the beta strands ABED and A'GFCC' form two beta sheets that are linked by a disulfide bond. In contrast to the demonstrated aggregation of Ig III on solid supports, we were unable to demonstrate self-association of Ig III under any of a variety of solution conditions. The structure shows that the surface of Ig III is dominated by two large acidic patches, which may explain our failure to observe self-association in solution. To evaluate the involvement of the Ig I-Ig II interaction in cell-cell adhesion, we designed a point mutation in Ig I (F19S) that proved sufficient to abrogate the Ig I-Ig II interaction seen in solution. However, the introduction of this mutation into full-length N-CAM expressed in COS-7 cells failed to affect N-CAM-mediated cell-cell adhesion. The inability to observe Ig III self-association in solution, combined with the failure of the F19S mutation to affect N-CAM-mediated cell-cell adhesion, suggests that, although solution studies can give important insights into the structures of individual domains, the interactions observed in solution between the domains may not be representative of the interactions that occur on the cell surface.

PubMed: 11469865
DOI: 10.1006/jmbi.2001.4861

実験手法

SOLUTION NMR