1IE2

Solution Structure of an In Vitro Selected RNA which is Sequence Specifically Recognized by RBD12 of Hamster Nucleolin.sNRE (anti)

1IE2 の概要

• エントリーDOI: 10.2210/pdb1ie2/pdb
• 関連するPDBエントリー: 1FJ7 1FJC 1FJE 1IE1
• 分子名称: 5'-R(*GP*GP*CP*CP*GP*AP*AP*AP*UP*CP*CP*CP*GP*AP*AP*GP*UP*AP*GP*GP*CP*C)-3' (1 entity in total)
• 機能のキーワード: eucaryotic loop e motif a form rna flexible loops, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7095.32

構造登録者

Bouvet, P.,Allain, F.H.-T.,Finger, L.D.,Dieckmann, T.,Feigon, J. (登録日: 2001-04-05, 公開日: 2001-06-20, 最終更新日: 2024-05-22)

主引用文献

Bouvet, P.,Allain, F.H.,Finger, L.D.,Dieckmann, T.,Feigon, J.
Recognition of pre-formed and flexible elements of an RNA stem-loop by nucleolin.
J.Mol.Biol., 309:763-775, 2001

PubMed Abstract: Nucleolin is an abundant nucleolar protein which is essential for ribosome biogenesis. The first two of its four tandem RNA-binding domains (RBD12) specifically recognize a stem-loop structure containing a conserved UCCCGA sequence in the loop called the nucleolin-recognition element (NRE). We have determined the structure of the consensus SELEX NRE (sNRE) by NMR spectroscopy. In both the free and bound RNA the top part of the stem forms a loop E (or S-turn) motif. In the absence of protein, the structure of the hairpin loop is not well defined due to conformational heterogeneity, and appears to be in equilibrium between two families of conformations. Titrations of RBD1, RBD2, and RBD12 with the sNRE show that specific binding requires RBD12. In complex with RBD12, the hairpin loop interacts specifically with the protein and adopts a well-defined structure which shares some of the features of the free form. The loop E motif also has specific interactions with the protein. Implications of these findings for the mechanism of recognition of RNA structures by modular proteins are discussed.

PubMed: 11397095
DOI: 10.1006/jmbi.2001.4691

実験手法

SOLUTION NMR