1IDV
NMR structure of HCV ires RNA domain IIIC
Summary for 1IDV
| Entry DOI | 10.2210/pdb1idv/pdb |
| NMR Information | BMRB: 5046 |
| Descriptor | HEPATITIS C IRES RNA DOMAIN IIIC (1 entity in total) |
| Functional Keywords | hepatitis c rna, ires, stem-loop, domain iiic, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 3207.97 |
| Authors | Kaluarachchi, K.,Rijnbrand, R.,Lemon, S.M.,Gorenstein, D.G. (deposition date: 2001-04-05, release date: 2001-10-05, Last modification date: 2024-05-22) |
| Primary citation | Rijnbrand, R.,Thiviyanathan, V.,Kaluarachchi, K.,Lemon, S.M.,Gorenstein, D.G. Mutational and structural analysis of stem-loop IIIC of the hepatitis C virus and GB virus B internal ribosome entry sites J.Mol.Biol., 343:805-817, 2004 Cited by PubMed Abstract: Translation of the open reading frames (ORF) of the hepatitis C virus (HCV) and closely related GB virus B (GBV-B) genomes is driven by internal ribosome entry site (IRES) elements located within the 5' non-translated RNA. The functioning of these IRES elements is highly dependent on primary and higher order RNA structures. We present here the solution structures of a common, critical domain within each of these IRESs, stem-loop IIIc. These ten-nucleotide hairpins have nearly identical sequences and similar overall tertiary folds. The final refined structure of each shows a stem with three G:C base-pairs and a novel tetraloop fold. Although the bases are buckled, the first and fourth nucleotides of both tetraloops form a Watson-Crick type base-pair, while the apical nucleotides are located in the major groove where they adopt C(2)-endo sugar puckering with B-form geometry. No hydrogen bonding interactions were observed involving the two apical residues of the tetraloop. Stability of the loops appears to be derived primarily from the stacking of bases, and the hydrogen bonding between the fourth and seventh residues. Mutational analysis shows that the primary sequence of stem-loop IIIc is important for IRES function and that the stem and first and fourth nucleotides of the tetraloop contribute to the efficiency of internal ribosome entry. Base-pair formation between these two positions is essential. In contrast, the apical loop nucleotides differ between HCV and GBV-B, and substitutions in this region of the hairpin are tolerated without major loss of function. PubMed: 15476802DOI: 10.1016/j.jmb.2004.08.095 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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