1ICO

SOLUTION STRUCTURE OF DESIGNED BETA-SHEET MINI-PROTEIN TH10BOX

1ICO の概要

• エントリーDOI: 10.2210/pdb1ico/pdb
• 関連するPDBエントリー: 1IC9 1ICL
• 分子名称: TH10BOX (1 entity in total)
• 機能のキーワード: de novo protein design three-stranded beta-sheet mini-protein motif th motif, de novo protein
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3268.76

構造登録者

Ottesen, J.J.,Imperiali, B. (登録日: 2001-04-02, 公開日: 2001-04-11, 最終更新日: 2024-10-30)

主引用文献

Ottesen, J.J.,Imperiali, B.
Design of a discretely folded mini-protein motif with predominantly beta-structure.
Nat.Struct.Biol., 8:535-539, 2001

PubMed Abstract: Here we report the creation of a predominantly beta-structured mini-protein motif. The design target is based on the naturally occurring toxin hand (TH) motifs that are composed of four disulfide bonds and three loops that form a 'hand'. Analysis and subsequent modification of several generations of mini-proteins produced the final 29-residue mini-protein. The structured motif of this new mini-protein provides insight into the compensatory changes that result in the formation of a tightly packed hydrophobic core in a small, globular beta-structure motif. Additionally, this mini-motif represents a new, distinct surface topology for protein design and a valuable, yet compact, model system for the study of beta-sheet structure in water.

PubMed: 11373623
DOI: 10.1038/88604

実験手法

SOLUTION NMR