1IC9

NMR SOLUTION STRUCTURE OF THE DESIGNED BETA-SHEET MINI-PROTEIN TH10AOX

Summary for 1IC9

• Entry DOI: 10.2210/pdb1ic9/pdb
• Related: 1ICL 1ICO
• Descriptor: TH10AOX (1 entity in total)
• Functional Keywords: three stranded antiparallel beta-sheet mini-protein motif de novo protein design, de novo protein
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 3282.79

Authors

Ottesen, J.J.,Imperiali, B. (deposition date: 2001-03-30, release date: 2001-04-11, Last modification date: 2024-10-30)

Primary citation

Ottesen, J.J.,Imperiali, B.
Design of a discretely folded mini-protein motif with predominantly beta-structure.
Nat.Struct.Biol., 8:535-539, 2001

PubMed Abstract: Here we report the creation of a predominantly beta-structured mini-protein motif. The design target is based on the naturally occurring toxin hand (TH) motifs that are composed of four disulfide bonds and three loops that form a 'hand'. Analysis and subsequent modification of several generations of mini-proteins produced the final 29-residue mini-protein. The structured motif of this new mini-protein provides insight into the compensatory changes that result in the formation of a tightly packed hydrophobic core in a small, globular beta-structure motif. Additionally, this mini-motif represents a new, distinct surface topology for protein design and a valuable, yet compact, model system for the study of beta-sheet structure in water.

PubMed: 11373623
DOI: 10.1038/88604

Experimental method

SOLUTION NMR