1IAW

CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA

1IAW の概要

• エントリーDOI: 10.2210/pdb1iaw/pdb
• 関連するPDBエントリー: 1ev7
• 分子名称: 5'-D(*TP*GP*CP*CP*AP*CP*GP*CP*CP*GP*GP*CP*GP*TP*GP*GP*C)-3', TYPE II RESTRICTION ENZYME NAEI (3 entities in total)
• 機能のキーワード: protein-dna complex, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Lechevalieria aerocolonigenes
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 91589.46

構造登録者

Huai, Q.,Colandene, J.D.,Topal, M.D.,Ke, H. (登録日: 2001-03-23, 公開日: 2001-08-03, 最終更新日: 2024-04-03)

主引用文献

Huai, Q.,Colandene, J.D.,Topal, M.D.,Ke, H.
Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement.
Nat.Struct.Biol., 8:665-669, 2001

PubMed Abstract: NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide.

PubMed: 11473254
DOI: 10.1038/90366

実験手法

X-RAY DIFFRACTION (2.4 Å)