1IAP

CRYSTAL STRUCTURE OF P115RHOGEF RGRGS DOMAIN

Summary for 1IAP

• Entry DOI: 10.2210/pdb1iap/pdb
• Related: 1AGR 1CMZ 1DK8 1FQJ
• Descriptor: GUANINE NUCLEOTIDE EXCHANGE FACTOR P115RHOGEF (2 entities in total)
• Functional Keywords: p115, rhogef, rgs, rgrgs, signaling protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 24276.92

Authors

Sprang, S.R.,Chen, Z. (deposition date: 2001-03-22, release date: 2001-09-05, Last modification date: 2024-04-03)

Primary citation

Chen, Z.,Wells, C.D.,Sternweis, P.C.,Sprang, S.R.
Structure of the rgRGS domain of p115RhoGEF.
Nat.Struct.Biol., 8:805-809, 2001

PubMed Abstract: p115RhoGEF, a guanine nucleotide exchange factor for Rho GTPase, is also a GTPase activating protein (GAP) for G(12) and G(13) heterotrimeric G alpha subunits. Near its N-terminus, p115RhoGEF contains a domain (rgRGS) with remote sequence identity to RGS (regulators of G protein signaling) domains. The rgRGS domain is necessary but not sufficient for the GAP activity of p115RhoGEF. The 1.9 A resolution crystal structure of the rgRGS domain shows structural similarity to RGS domains but possesses a C-terminal extension that folds into a layer of helices that pack against the hydrophobic core of the domain. Mutagenesis experiments show that rgRGS may form interactions with G alpha(13) that are analogous to those in complexes of RGS proteins with their G alpha substrates.

PubMed: 11524686
DOI: 10.1038/nsb0901-805

Experimental method

X-RAY DIFFRACTION (1.9 Å)