1IAI

IDIOTYPE-ANTI-IDIOTYPE FAB COMPLEX

Summary for 1IAI

• Entry DOI: 10.2210/pdb1iai/pdb
• Descriptor: IDIOTYPIC FAB 730.1.4 (IGG1) OF VIRUS NEUTRALIZING ANTIBODY, ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A), ... (4 entities in total)
• Functional Keywords: complex (immunoglobulin igg1-igg2a), complex (immunoglobulin igg1-igg2a) complex, complex (immunoglobulin igg1/igg2a)
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 4
• Total formula weight: 94887.29

Authors

Ban, N.,Escobar, C.,Garcia, R.,Hasel, K.,Day, J.,Greenwood, A.,McPherson, A. (deposition date: 1993-12-28, release date: 1996-03-08, Last modification date: 2024-10-16)

Primary citation

Ban, N.,Escobar, C.,Garcia, R.,Hasel, K.,Day, J.,Greenwood, A.,McPherson, A.
Crystal structure of an idiotype-anti-idiotype Fab complex.
Proc.Natl.Acad.Sci.USA, 91:1604-1608, 1994

PubMed Abstract: Anti-idiotypic monoclonal antibody 409.5.3 is raised against an antibody that neutralizes feline infectious peritonitis virus. This antibody, used as an immunogen, elicits the production of anti-anti-idiotypic antibodies that in turn neutralize the virus. The crystal structure of the complex between anti-idiotypic Fab 409.5.3 and idiotypic Fab fragment of virus-neutralizing antibody has been solved by molecular replacement using real-space Patterson search and filtering by Patterson correlation-coefficient refinement. The structure has been refined to an R value of 0.21 based on 21,310 unique reflections between 40.0 and 2.9 A. The three-dimensional structure reveals extensive, specific interactions that involve 118 van der Waals contacts and at least 9 probable hydrogen bonds. The two Fabs are rotated 61 degrees with respect to each other around the approximate long axis of the complex and are within 26 degrees being aligned along their major axes.

PubMed: 8127852
DOI: 10.1073/pnas.91.5.1604

Experimental method

X-RAY DIFFRACTION (2.9 Å)