1I9E

TCR DOMAIN

1I9E の概要

• エントリーDOI: 10.2210/pdb1i9e/pdb
• 分子名称: CYTOTOXIC TCELL VALPHA DOMAIN, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: ig-like domain, t cell receptor, immune system
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12995.54

構造登録者

Rudolph, M.G.,Huang, M.,Teyton, L.,Wilson, I.A. (登録日: 2001-03-19, 公開日: 2001-12-05, 最終更新日: 2024-10-30)

主引用文献

Rudolph, M.G.,Huang, M.,Teyton, L.,Wilson, I.A.
Crystal structure of an isolated V(alpha) domain of the 2C T-cell receptor.
J.Mol.Biol., 314:1-8, 2001

PubMed Abstract: The T-cell receptor (TCR) is a heterodimeric cell-surface protein consisting of two chains, alpha and beta, each of which is composed of a variable (V) and a constant (C) domain. Crystals of the isolated V(alpha) domain of the murine TCR 2C were grown by serendipity from a solution containing the extracellular domains of the intact TCR 2C and CD3 gamma epsilon-chains. The V(alpha) crystal structure shows how crystal packing can substitute for another V(alpha) domain in a different fashion from that observed in V(alpha)/V(alpha) homodimer and V(alpha)/V(beta) heterodimer structures. Significant conformational changes occur in the CDR3 and beta(3)beta(4) loops that normally form part of the dimer interface. The monomeric V(alpha) domain provides the unique opportunity to study the effect of dimerization on the conformation of the unliganded complementarity-determining regions (CDR) of a TCR. This structure of an individual V(alpha) module has implications for stability and bioengineering of isolated antibody and immunoglobulin domains.

PubMed: 11724527
DOI: 10.1006/jmbi.2001.5113

実験手法

X-RAY DIFFRACTION (2.5 Å)